EVALUATING THE SIGNIFICANCE OF ACTINOMYCETES INFECTIONS IN TB SMEAR NEGATIVE AND RETREATMENT CASES FROM SELECTED REFERRAL FACILITIES KENYA.
Introduction:
Actinomycetes are opportunistic pathogens causing infections in patients with immunosuppressive conditions.rnPulmonary infections are common though cutaneous diseases also occur. In most cases respiratory infections caused byrnActinomycetes display symptoms that mimic tuberculosis hence they can easily be misdiagnosed as TB leading to delayed orrninappropriate treatment. Therefore this study aimed at evaluating the significance of Actinomycetes infections in TB smearrnnegative and retreatment patients from selected referral facilities in Kenya.
Objectives:
NOT STATED
Methodology:
This was a cross-sectional laboratory based study where purposive sampling method was used. The samples wererncollected from 4 sites ie Moi Teaching and Referral Hospital Jaramogi Oginga Odinga Teaching and Referral Hospital CoastrnProvincial General Hospital and Mbagathi Hospital. Sputum samples 385 from consented TB smear negative and retreatmentrnpatients were collected and screened for Actinomycetes. This was done via phenotypic and genotypic characterization. Phenotypicrncharacterization involved gram staining culturing and biochemical testing. Confirmatory identification was done by molecularrnmethods PCR and Sequencing where DNA was extracted and amplified using primers that target 16S rRNA gene.
Findings:
Results:
Out of the collected samples 52 13.5 were identified as Actinomycetes using the phenotypic methods. The 16S rRNArngene was successfully amplified in 32 8.3 of the isolates. Sequencing was done and Phylogenetic analysis confirmed that ninern2.3 were members of Actinomycetes of which eight were members of the genus Streptomyces while one was a Nocardiopsisrnspecies.
Conclusion:
There is significant Actinomycetes infections in TB smear negative and retreatment patients with a prevalence ofrn13.5. Retreatment cases had more actinomycetes infections than new cases JOOTRH had highest cases while CPGH had thernleast. Most of the isolates were found to be from the genus Streptomyces. Therefore it is important to investigate suspectedrnpulmonary pathologies for potential Actinomycetes infection for proper diagnosis and interventions
Publication Information
Author(s):
Focus County(s):
National
Programme Area(s):
Infectious and Parasitic Diseases
Research Priority Area(s):
Disease Domain(s):
TB
Document History:
Publication Date: 15.Feb.2022
Conference Title:
Venue: