Antimicrobial resistance and virulence characteristics of Klebsiella pneumoniae isolates in Kenya
Introduction:
Klebsiella pneumoniae K. pneumoniae is a globally significant opportunistic pathogen associated with healthcare-acquired and community-acquired infections. This study examined the epidemiology and the distribution of resistance and virulence genes in clinical K. pneumoniae strains in Kenya.
Objectives:
Examining the epidemiology and the distribution of resistance and virulence genes in clinical K. pneumoniae strains in Kenya.
Methodology:
Eighty nine K. pneumoniae isolates were collected over six years from five Counties in Kenya and were analyzed using whole genome sequencing and bioinformatics.
Findings:
Genetic analysis revealed the presence of blaNDM-1 and blaOXA-181 carbapenemase genes as well as armA and rmtF genes known to confer pan-aminoglycoside resistance. The most abundantrnextended-spectrum beta-lactamase genes identified were blaCTX-M-15 36/89 blaTEM 35/89 and blaOXA 18/89. Onernisolate had a mobile colistin resistance gene mcr-8. Fluoroquinolone resistance-conferring mutations in gyrA and parC genesrnwere also observed. The most notable virulence factors were those associated with hyper-virulence rmpA/A2 and magArnyersiniabactin ybt salmochelin iro and aerobactin iuc and iutA. Thirty-eight distinct sequence types were identified includingrnknown global lineages ST14 ST15 ST147 and ST307 and a regional clone ST17 implicated in regional outbreaks.
Results:
There were two potential hypervirulent isolates and two community-acquired ST147 high-risk clones that contained carbapenemase genes yersiniabactin and other multi-drug resistance genes.
Conclusion:
These results demonstrate that the resistome and virulome of Kenyan clinical and hospital environmental K.rnpneumoniae isolates are diverse and that there is a reservoir of high risk-clones capable of spreading resistance and virulencernfactors which have the potential to cause unmanageable infection outbreaks with high morbidity and mortality.
Publication Information
Author(s):
Focus County(s):
National
Programme Area(s):
Biotechnology
Research Priority Area(s):
Disease Domain(s):
Document History:
Publication Date: 01.Jan.1970
Conference Title:
12 KASH th Conference
Venue:
SAFARI PARK HOTEL NAIROBI IN PERSON VIRTUAL